Correlative Light and Electron Microscopy Using Frozen Section Obtained Using Cryo-Ultramicrotomy

Immuno-electron microscopy (Immuno-EM) is a powerful tool for identifying molecular targets with ultrastructural details in biological specimens. However, technical barriers, such as the loss of ultrastructural integrity, the decrease in antigenicity, or artifacts in the handling process, hinder the widespread use of the technique by biomedical researchers. We developed a method to overcome such challenges by combining light and electron microscopy with immunolabeling based on Tokuyasu’s method. Using cryo-sectioned biological specimens, target proteins with excellent antigenicity were first immunolabeled for confocal analysis, and then the same tissue sections were further processed for electron microscopy, which provided a well-preserved ultrastructure comparable to that obtained using conventional electron microscopy. Moreover, this method does not require specifically designed correlative light and electron microscopy (CLEM) devices but rather employs conventional confocal and electron microscopes; therefore, it can be easily applied in many biomedical studies.     

基于结构光的星箭对接环相对位姿测量方法

空间非合作目标位姿测量是空间在轨维护的前提。面向空间机械手对空间载体自动抓捕的应用需求，提出了基于结构光的空间非合作目标视觉测量方法。该方法以双套双线结构光测量装置作为测量传感器，以空间载体上普遍存在的星箭对接环作为抓捕目标。根据对接环的共同特点，选择直线特征和点特征相结合作为相对位姿的求解特征；基于直线特征求解圆环平面法向量，基于点特征求解圆环圆心坐标，进而得到机械手工具坐标系与对接环坐标系间的相对位姿关系；基于多重几何约束实现了不同光照条件下图像上目标直线的鲁棒识别。建立了演示验证实验系统，在大量演示实验的基础上，进行了实验结果分析。     

Synthesis and spectroscopic studies of some new azobenzenesulfonamide

Abstract

Diazotized sulfadiazine derivative (1) was coupled with some nitrogen heterocyclic namely (thiazolone derivatives 2a–c, thiazole derivatives 4a–c and barbituric acid derivatives 6a,b) to give the corresponding azo compounds 3a–c, 5a–c and 7a,b in good yields. The newly synthesized azo benzene sulfonamide dyes were recorded their UV visible spectra in the region 200–600?nm in four different solvents and discussed the effect of solvents, substituent and effect of acid and base on the absorption values of these dyes.     

以用户为中心的可见光通信协作传输中训练资源分配算法

以用户为中心的可见光通信协作传输是近年来出现的新架构,这导致虚拟小区之间出现重叠。为避免导频污染问题,每个虚拟小区中的光接入点(AP)或者虚拟小区中选择相同AP的用户发送的训练序列应该是正交的。针对可见光通信中以用户为中心的协作网络,研究训练资源的正交分配问题,提出了一种新的导频分配算法,联合导频分配和用户选择问题,以期最大限度地增加虚拟小区内可被接入的用户数。分析和仿真结果表明,该导频分配方案可以有效改善导频污染问题,提高训练资源利用率,并且相比已有的导频分配方案,性能有所改进。     

Development of an innovative apparatus using UV‐C for controlling the number of microorganisms in raw milk after milking

This study was conducted to investigate the effect of UV‐C irradiation on the reduction of microorganisms in raw milk. The experiments were carried out by varying the flow rates of 2, 4 and 7 L/min with 39W and 48W UV‐C. The number of microorganisms was reduced by 4.70 (39W) and 4.60 log₁₀ CFU/mL (48W) at 120 min. With the residence time of 4.95 s and the UV‐C doses of 98 and 109.9 mJ/cm² at 253.74 nm, the reduction of microorganism was clearly observed with no significant influence of different UV‐C power settings on the oxidation of milk fat.     

不同LED光质对绿叶生菜生长的影响

选用半结球生菜和散叶生菜两种绿叶生菜为试材,在其他生长条件相同的情况下,设置3个红蓝光配比,分别为R∶B=4∶1、R∶B=3∶1、R∶B=1∶1,探究不同LED光质对绿叶生菜生长的影响。结果表明:半结球生菜在株高、横径、叶片数、单株鲜重、地上部鲜重方面均随着红光的增多呈上升趋势,并在R∶B=4∶1下达到最大,而地下部鲜重和根冠比在R∶B=1∶1下达到最大;散叶生菜的株高、横径、叶片数、单株鲜重和地上部鲜重均在R∶B=3∶1下达到最大。结论:在本试验设置的3种光配比中,R∶B=4∶1最有利于半结球生菜的生长,R∶B=3∶1最有利于散叶生菜的生长。     

Ternary adsorbent photocatalyst hybrid (APH) nanomaterials for improved abstraction of tetracycline from water

ABSTRACT

Current health situations have instigated increased frequency of taking antibiotics for cure of infections but this amplified use is posing threats to environment. This research is focused to remove Tetracycline (TC), an antibiotic from water, using an advanced nanohybrid that compact the properties of adsorbent and photocatalyst. Compared to conventional methods for remediation of TC, large surface area (452 m²g^?1) adsorbent photocatalyst hybrid (APH) g-C₃N₄/α-MoO₃/ZIF-67 (CMZ) have found much effective as 97% degradation of TC is observed in 110 min with 0.1 g of APH. Increasing g-C₃N₄ in the hybrids has improved percent degradation of TC molecules. CMZ-3 is found as a potential candidate for water treatment.     

Fabrication of magnetically recoverable Fe3O4/CdS/g-C3N4 photocatalysts for effective degradation of ciprofloxacin under visible light

Photocatalytic technology is an environmentally safe method of eliminating organic pollutants and antibiotics in wastewater. In this research, the performance of Fe₃O₄/CdS/g-C₃N₄ (FCN) photocatalyst for degradation of antibiotics was studied. The composite photocatalysts with different concentrations of g-C₃N₄ were prepared. FCN has better photocatalytic activity than degradation dyes in removal of antibiotics under visible light. This indicates that FCN could effectively hinder the recombination of carriers, and the addition of g-C₃N₄ increases the optical response range of CdS. At the same time, the introduction of Fe₃O₄ magnetic nanoparticles overcomes the problem of difficulty in recovery of the powder photocatalyst. The photocatalytic activity is not reduced to any significant after three cycles of use.